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1.
患病罗汉鱼疑似病原菌分离鉴定与药敏试验 总被引：2，自引：0，他引：2
[目的]分离鉴定患病罗汉鱼疑似病原菌,并进行药敏试验。[方法]对患病罗汉鱼进行体表、口腔、鳃、肾、肝、肠等器官及肌肉组织的病理变化观察。在病灶（下颌）、肾、肝、鳃处分离细菌,并对分离的细菌进行初步鉴定和药敏试验。[结果]在病灶（下颌）、肾、肝、鳃处分离出3株优势细菌。通过菌落形态观察以及生理生化测定,鉴定出该3株细菌分别为嗜水气单胞菌（Aeromonas hydrophila）、假单胞菌（Pseudomonas sp.）、链球菌（Streptococcus sp.）,适宜盐度分别为：10.00‰~20.00‰0、.01‰~1.00‰、0.01‰~1.00‰,对四环素、红霉素、土霉素等药物高度敏感。[结论]为找到预防与治疗罗汉鱼疾病的有效手段提供了科学依据,同时也为其他观赏鱼细菌性疾病的防治提供参考和借鉴。 相似文献
2.
试验研究了在基础饲料中添加加丽素红、维生素E、磷脂的混合物对血鹦鹉鱼(Cichlasoma synspilum×Cichlasoma citrinellum)体色和生理指标的影响。试验设3个处理,分别为对照组(处理A),0.7%加丽素红与0.3%维生素E组(处理B),0.7%加丽素红与0.3%磷脂组(处理C),养殖30 d,测定血鹦鹉鱼体内超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、类胡萝卜素、谷丙转氨酶(GPT)及谷草转氨酶(GOT)等指标。结果表明,处理B血鹦鹉鱼亮度和红度显著高于其他组(P0.05);处理B终体重、增重率、特定生长率显著高于其他组(P0.05)。3个处理间血鹦鹉鱼肝胰脏、肌肉和皮肤中类胡萝卜素含量差异不显著。血鹦鹉鱼肝胰脏中,处理B SOD、CAT活性显著高于其他组(P0.05),处理B MDA活性显著低于其他组(P0.05)。3个处理血清中GOT和GPT含量差异不显著(P0.05)。在基础饲料中添加0.7%加丽素红与0.3%维生素E对鹦鹉鱼的体色、生长和抗氧化系统效果较好。 相似文献
3.
马拉瓜丽体鱼(Cichlasoma managuense)水泥池人工繁殖与育苗技术试验结果表明：在网箱内培育的2龄亲鱼可在水泥池中自行配对产卵。雌鱼绝对产卵量为1 500～2 500粒/尾，相对产卵量500～800 ind /kg。受精卵为椭圆形，暗黄色，不透明，粘性，卵分散排列在巢穴中。试验期间，70对亲鱼中有52对自行配对产卵，产卵率为74.2%，受精率为92.6%。在水温27～30 ℃时，从受精卵到破膜时间为48 h±41min，出膜仔鱼全长(0.65±0.35)cm，孵化率为84.8%，共获初孵仔 相似文献
4.
马拉瓜丽体鱼(Cichlasoma managuense)水泥池人工繁殖与育苗技术试验结果表明:在网箱内培育的2龄亲鱼可在水泥池中自行配对产卵。雌鱼绝对产卵量为1 500～2 500粒/尾,相对产卵量500～800 ind/kg。受精卵为椭圆形,暗黄色,不透明,粘性,卵分散排列在巢穴中。试验期间,70对亲鱼中有52对自行配对产卵,产卵率为74.2%,受精率为92.6%。在水温27～30℃时,从受精卵到破膜时间为48 h±41min,出膜仔鱼全长(0.65±0.35)cm,孵化率为84.8%,共获初孵仔鱼40.1万尾。在水泥池中用小体积网箱进行苗种培育,经41～43 d的培育共获得3.0～5.0 cm鱼苗32.2万尾,育苗成活率达78.5%。 相似文献
5.
Partial characterization of digestive proteases of the three‐spot cichlid Cichlasoma trimaculatum (Günter 1867) 
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下载免费PDF全文 F.J. Toledo‐Solís G. Márquez‐Couturier A. Uscanga‐Martínez R. Guerrero‐Zárate N. Perales‐García R. Martínez‐García W.M. Contreras‐Sánchez S. Camarillo‐Coop C.A. Álvarez‐González 《Aquaculture Nutrition》2016,22(6):1230-1238
Partial characterization of digestive proteases in the three‐spot cichlid Cichlasoma trimaculatum juveniles was conducted. It was determined that there is higher alkaline proteases activity (3.95 ± 0.32 IU mg?1 protein) compared to acidic proteases (2.01 ± 0.57 IU mg?1 protein). Optimal temperature for alkaline proteases is 60 °C which resulted in more thermostability to temperature changes. On the other hand, optimal temperature for acidic proteases is 50 °C. Optimal pH for acidic proteases was pH 2, while for alkaline proteases, it was pH 10, which resulted in more stability in relation to pH changes than acidic protease. The use of specific inhibitors and the SDS‐PAGE electrophoresis analysis revealed seven types of bands for alkaline proteases, which make evident the main presence of serine proteases. In acidic proteases, more than 98 g kg?1 of the activity was inhibited with pepstatin A inhibitor. Therefore, it is evident that C. trimaculatum digestion is composed by acidic and alkaline proteases; thus, it should be considered an omnivorous fish. 相似文献
6.
The long term (two generations) effect an enhanced (5%) sub-lethal ultraviolet-A (320–400 nm) irradiation on growth and survival rate of a convict cichlid (Cichlasoma nigrofasciatum is presented. Two UVA irradiated populations (G1-UVA and G2-UVA) and two control populations (G1-control and G2-control) were observed from age 60 to 403 days. The UVA populations were exposed to enhanced UVA irradiation from 4 months of age (G1-UVA and from egg's state (G2-UVA).The specific growth rates (daily weight and length increase) and the condition factors of the populations were not affected by the enhanced UVA, it depended more on the population size. At the adapted temperature range (27–29 °C) the survival rate of those populations was not affected by enhanced UVA. A combined effect of enhanced UVA and increased temperature (31–32 °C) was responsible for a high mortality rate in the GI-UVA. A total of 67% of the G1-UVA population died just within a week of exposure to high temperature. 相似文献
7.
Effects of supplementary fennel (Foeniculum vulgare) essential oil in diet on growth and reproductive performance of the ornamental fish,Convict cichlid (Cichlasoma nigrofasciatum) 下载免费PDF全文
下载免费PDF全文 This study evaluated the effects of diet containing 0 (C: control), 75 (D1), 100 (D2), 125 (D3) and 150 (D4) mg kg?1 F. vulgare essential oil on growth and reproductive performance of C. nigrofasciatum. A total of 225 Convict cichlids female with mean weight (1.65 ± 0.02 g) were distributed into 15 glass aquaria (15 fish tank?1) in triplicate treatments. During 40 days of experimental period, fish fed at 3% of their body weight daily. At the end of experiment, growth indices, gonadosomatic index and reproductive indices were determined. The best feed conversion ratio was obtained in D4 (1.19 ± 0.03, P < 0.05). The addition of F. vulgare essential oil did not have any statistical effects on other growth indices (specific growth rate, weight gain and condition factor). Total survival rates in all treatments and control were uniformly high, ranging from 93% to 97% (P > 0.05). F. vulgare essential oil has no negative effect on survival rate of C. nigrofasciatum. There were no significant differences among protein content of fish body (P > 0.05). The lowest value of fat content (26.5 ± 0.5%) was observed in D2, and it had significant difference with control and other groups (P < 0.05). The ash percentage in treatments fed with F. vulgare essential oil were higher than control fish (P < 0.05). The highest moisture content was detected in D4 (72.68 ± 0.19%) and D2 (71.23 ± 2%) groups. Among the used dosage, D4 was the most effective dosage that could significantly increase GSI (11.06 ± 1.55%), fecundity (340 ± 21) and hatching ratio (92.33 ± 1.63%). There were no significant differences in diameter of eggs among various treatments (P > 0.05). 相似文献
8.
淡水石斑鱼和美国豹纹牛蛙蝌蚪对 Zn~(2+)的富集倍数随锌浓度增加而降低,各组织器官对锌富集能力的大小顺序为:内脏>鳃>去内脏体。Zn~(2+)对两者的半致死浓度(mg/l)分别为:24h:165.00与130.00,48h:150.00与110.00,96h:77.98与70.00mg/l,安全浓度分别为0.78与0.70mg/1. 相似文献
9.
We have examined the effects of Tribulus terrestris (TT), a non-toxic herb, on sex reversal in the Convict Cichlid Cichlasoma nigrofasciatum with the aim of introducing a new environmentally friendly method for masculinization in C. nigrofasciatum. TT is a natural plant product that elevates the testosterone levels in humans and animals. Different concentrations (0.0,
0.10, 0.20 and 0.30 g/l) of TT extract were tested for their effect on sex reversal in C. nigrofasciatum by immersing newly hatched offspring once weekly for 2 months in TT extract. Of the dosages used in the present study, 0.30 g/l
TT was the most effective in terms of masculinization, resulting in a maximum male ratio of 87.23% (P < 0.001). Sex ratios of 79 and 85% at 0.10 and 0.20 g/l TT, respectively, were also significantly different from the expected
1:1 ratio (P < 0.001). Histological examinations revealed that the testes of fish treated with TT extract contained all stages of spermatogenesis,
clearly demonstrating that the administration of TT extract to C. nigrofasciatum stimulated spermatogenesis. Total survival rates in all treatments and the control were uniformly high, ranging from 88.57
to 90% (P > 0.05). We concluded that TT had no negative effect on the survival rate of C. nigrofasciatum. In addition, all groups of TT-treated fish exhibited successful growth acceleration compared to the control group, but only
the 0.30 g/l TT treatment significantly improved the growth rate of C. nigrofasciatum. (P < 0.01). Sex reversal in C. nigrofasciatum demonstrated that TT-treated 0-day-old larvae showed successful sex reversal, spermatogenesis and a better growth rate than
untreated progenies. 相似文献
10.
研究了口服甲基睾丸酮(MT)诱导马拉瓜丽体鱼(Cichlasoma managuense)雄性化效应与鱼苗规格、剂量和处理时间的关系,并讨论了MT对马拉瓜丽体鱼的生长率和成活率的影响。结果表明,MT诱导1.2 cm以下鱼苗可获得较高的雄性率;鱼苗的雄性率随着MT含量增加和处理时间延长而提高,投喂剂量为40 mg/kg的饲料30d、50 mg/kg的饲料20 d以上,或者60 mg/kg的饲料10 d以上,鱼苗的雄性率均可达到90%以上;MT能提高马拉瓜丽体鱼的生长率,而与成活率没有直接依赖关系。 相似文献